ASCB Rainbow

Subgroup A

Subgroup A: 4D Nucleome Organization: Unlocking the structure-function relationships of genome organization and nuclear morphology

8:30 am – 12:30 pm, Room 108B
Organizers: Sean E. Hanlon, National Cancer Institute; and Andrew Stephens, Northwestern University

The nucleus is dynamically organized across length-scales, from genome architecture to nuclear body organization and overall nuclear morphology. All of these features influence gene expression and overall cellular function. For decades alterations to genome architecture and nuclear morphology have been observed in healthy cells or used as diagnostic markers of disease but the mechanistic details underlying these alterations remain unknown.  In 2015, the NIH Common Fund launched the 4D Nucleome program to investigate principles underlying nuclear organization, the role nuclear organization plays in cellular function, and how altering nuclear organization affects development and disease. This session will highlight recent advances in understanding genome and nuclear organization using approaches from genomics, biophysics and biomechanics, imaging, visualization, and computational modeling.


8:30-8:40 am- Introduction. Sean E. Hanlon, National Cancer Institute
8:40-9:10 am- Chromatin histone modifications and rigidity affect nuclear morphology independent of lamins. Andrew Stephens, Northwestern University
9:10-9:40 am- Role of nuclear lamina assembly in nuclear envelope rupture and repair. Emily Hatch, Fred Hutchinson Cancer Research Center
9:40-10:10 am- The mechanics of nuclear shaping. Tanmay Lele, University of Florida
10:10-10:40 am- Probing chromatin mechanics by applying diffusive state analysis to living cells. Megan King, Yale University
10:40-11:00 am- Break
11:00-11:30 am- Spatiotemporal Control of Intracellular Phase Transitions Using Light-Activated optoDroplets. Clifford Brangwynne, Princeton University
11:30 am-12:00 pm- 3D Epigenome reconfiguration in brain development and disease. Jennifer Phillips-Cremins, University of Pennsylvania
12:00-12:30 pm – Exploring long-range genome interaction data using the WashU Epigenome Browser. Ting Wang, Washington University, St. Louis

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