ASCB Rainbow


Exhibitor Tech Talks

SUNDAY, DECEMBER 3, 2017MONDAY, DECEMBER 4, 2017TUESDAY, DECEMBER 5, 2017

8:15-9:15 am, Theater 2,  Learning Center
Thermo Fisher Scientific Inc.: Essential tools for fluorescent imaging, labeling, and detection
Presenter:  Oggie Golub, PhD
Level: Intermediate
The combination of light microscopy and fluorescent reporters offers an unparalleled view into the structure and function of intact cells. Invitrogen™ Molecular Probes™ reagents have been at the cutting edge of fluorescent reporter development for over four decades. In this seminar, we will review our portfolio of long-proven tools and protocols that have enabled researchers to confidently create publication quality cell images on the first attempt. Whether you’re new to cell imaging, or an experienced researcher wanting to use your imaging platform at its fullest potential, join us to learn about the latest innovations in fluorescence microscopy including high throughput imaging platforms, as well as reagents that reduce background fluorescence, amplify fluorescent signal output, prevent photobleaching , and improve spatial resolution of 3D samples.

9:30-10:30 am, Theater 1, Learning Center
Hybrigenics Corp: Efficient selection of single-domain antibodies from a naïve synthetic library using phage display and exhaustive yeast two-hybrid screening
Presenter: Petra Tafelmeyer
Level: Intermediate
High-affinity single-domain antibodies, like VHH, represent indispensable tools for research and clinical applications. To access VHH without Llama immunization, we have built a fully synthetic humanized naïve Llama VHH library containing 3x10exp9 antibodies, based on a unique scaffold with random complementary determining regions (CDRs). We have set up a new platform for phage display selection of VHH and successfully selected antibodies against a variety of antigens from large proteins to haptens, soluble proteins and receptors directly selected from cell surface expression. A combination with subsequent exhaustive yeast two-hybrid (Y2H) screening allows to further favor the selection of intrabodies – antibodies working inside living cells. We will exemplify the use of this library and our platform capabilities focusing on GFP, USP7 and Tau proteins as targets

10:45 am-11:45 am, Theater 1, Learning Center
GE Healthcare: Learn how GE’s DeltaVision microscopes can help your lab secure more funding
Presenters: Leanna Ferrand/Trisha Koenke
Level:  Intermediate
Securing future funding is on every researcher’s mind these days. Combined with the need to publish quickly and be confident in your findings – you either need to create more hours in a day or find a way to be more efficient with the resources you have. See how GE’s latest DeltaVision microscopes are revolutionizing lab productivity by providing reliable, accurate results faster than ever! This goes far beyond any technical specification sheet – it must also be usable by every level of researcher from novice to expert as well as stable, easy to maintain and capable of scaling with your research. Learn how the DeltaVision’s widefield deconvolution technology can advance your research among most cell biology applications, including live-cell imaging and high-throughput screening

10:45-11:45 am, Theater 2, Learning Center
BioLegend: Bone cell differentiation and bone cancer
Presenter: Mohar Chattopadhyay
Level: Intermediate
Osteoclasts and osteoblasts are specialized bone cells that are responsible for bone formation, repair, maintenance, and bone remodeling. Osteoclasts are derived from the monocyte/macrophage hematopoietic lineage, whereas osteoblasts arise from mesenchymal stem cells (MSC). Several cytokines and growth factors, including Receptor Activator of Nuclear Factor-kappaB Ligand (RANKL), Sonic Hedgehog family of proteins, and macrophage colony-stimulating factor (M-CSF), play critical roles in bone cell differentiation from precursor cells. Bone homeostasis depends on the strict balance between the bone formation activity of osteoblasts and bone resorption by osteoclasts. Any imbalance in this system can result in various bone diseases. As both primary bone tumors, such as osteosarcoma, and secondary tumors (metastases), can develop in the bone, it is therefore also necessary to understand the biology in malignant bone environment. Here we present our portfolio, validation methods, and data exploring bone cell differentiation and bone cancer using several assays, such as primary bone marrow cell differentiation to macrophages and further differentiation into osteoclasts using RANKL, osteoblast differentiation, cancer cell invasion, chromatin immunoprecipitation (ChIP), magnetic cell separation, and target-ligand inhibition bioassays, among others. We also explored intra-cellular signaling pathways and mitochondrial dysfunction in osteosarcoma cells

12:00-12:45 pm, Theater 1, Learning Center
Carl Zeiss Microcopy, LLC: Robust label free cell proliferation measurement with Celldiscoverer 7 and machine learning based image processing
Presenter: Dr. Christoph Moehl
Level: Advanced
Label-free cell proliferation assays based on contrast imaging can be easily applied on an automated microscopy setup to test toxicity of compounds. No additional assay development steps such as reporter-systems or stably transfected cell models need to be established and the same assay approach can be introduced for several cell models with low effort. However, the image analysis of transmitted light contrast images is much more demanding than simple intensity thresholding, which is often sufficient for fluorescent micrographs. Here we present a robust workflow for cell proliferation measurements where images are taken at large scale with the Zeiss Celldiscoverer 7 and subsequently analyzed with big data analysis techniques based on supervised machine learning.

12:00-12:45 pm, Theater 2, Learning Center
Bruker Corporation: Harnessing the power of super-resolution single molecule localization microscopy with the Vutara 352: labeling and imaging strategies
Presenter: Manasa V. Gudheti, PhD
Level: Introductory
Single molecule localization microscopy (SMLM) has made a significant impact in the field of biology by enabling a 10-fold enhancement in resolution. Key factors in achieving this enhanced resolution is to optimally label and image the specimen. Numerous labeling strategies exist to tag structures in cells, bacteria, virus, tissue sections, C. elegans and Drosophila to make the best use of SMLM. Examples include DNA- and Oligo-Paint, antibody/nanobody labeling with organic dyes, Halo and SNAP-tag dyes, and photo-switchable fluorescent proteins. Choosing a sub-optimal labeling method for a given biological sample will result in loss of achievable resolution. Once a specimen has been optimally labeled and imaged, the acquired localization data can then be readily quantified via statistical analysis to test experimental hypotheses.

1:00-1:45 pm, Theater 1, Learning Center
Carl Zeiss Microcopy, LLC: Investigation of live cell dynamics with the Airyscan imaging technology
Presenter: Dr. B. Christoffer Lagerholm
Level: Advanced
The Airyscan technology, which combines confocal imaging with a 0.2 AU pinhole, deconvolution, and pixel-reassignment in order to enhance both the spatial resolution and signal-to-noise-ratio, was recently developed to cover the gap between conventional confocal and super-resolution microscopy. Here, we present comparative results of the performance of the Airyscan technology by imaging of a variety of reference and biological specimens. These results show that the spatial resolution of Airyscan images, processed at default deconvolution settings, are similar to confocal images with a 0.2 AU pinhole setting but with significantly improved signal-to-noise-ratio. Our results also show that further resolution gains, up to a point, are possible by careful manipulation of the deconvolution settings. Finally, we show applications demonstrating the Airyscan imaging compatibility with live cell applications.

1:00-1:45 pm, Theater 2, Learning Center
Andor Technology: 3D and 4D microscopy image analysis in cell biology – Imaris technology for analysis of large, complex datasets and visualization of multimodal images (e.g. fluorescence, EM, transmitted light)
Presenter: Meredith Price
Level: Intermediate
This session will focus on the improved technology for automated 3D/4D analysis of large microscopy images (100s GBs) and analysis tools for cell biologists including segmentation of cells, tissues or organs, tracking with cell division detection and system dynamics. Relevant applications include the following: tissue assembly and morphogenesis, nuclear organization, cell interactions, microtubule dynamics, neurobiology and cell migration. New possibilities for simultaneous visualization of multimodal images, including correlative light and electron microscopy, transmitted light or phase contrast will also be showcased.

2:00-2:45 pm, Theater 1, Learning Center
Allen Institute for Cell Science: The Allen Institute for Cell Science – resources to empower your research
Presenter: Allen Institute for Cell Science
Level: Introductory
We will introduce you to the publicly available data, tools, observations, methods, and cell lines produced by the Allen Institute for Cell Science. Learn about our legacy collection of endogenous fluorescently tagged human induced pluripotent stem cell lines highlighting key structures within the cell. See examples of, and how to navigate, our large, high replicate 3D image data sets showing the subcellular localization of each of these tagged structures, and hear about our microscopy pipeline. We will discuss instrumentation, automation, quality control, and 3D segmentations using the recently released CellProfiler 3.0. These data are integrated using deep neural networks to generate unified, integrated cell models. Take a guided tour through our website (http://www.allencell.org) and find out how our work can help you.

2:00-2:45 pm, Theater 2, Learning Center
Thermo Fisher Scientific Inc.: Thermo Scientific™ Amira Software© for Cell Biology, a new solution for interactive visualization and advanced analysis of 3D/4D image data
Presenter: Trevor Lancon
Level: Intermediate
Processing and analyzing experimental data to understand living cells and their processes can represent a unique challenge. During this talk we will present Amira Software for Cell Biology, which provides a comprehensive array of tools for the flexible and accurate analysis of time series data of cellular processes. Attendees will be able to discover the new tools and workflows for cell and particle tracking and analysis, filament tracing and editing, CLEM workflows, out-of-core volume management now supporting Bio-Formats, and also the flexible data import and new Python integration. Don’t miss our talk!

3:00-4:00 pm, Theater 2, Learning Center
GORYO Chemical, Inc.: Fluorescent probes for intra and extracellular biology
Presenter: Raj Singh, PhD
Level: Intermediate
Fluorescent probes are an essential suite of reagents to probe and elucidate extracellular as well as intracellular processes in biology. A number of unique, highly specific reactive oxygen species (ROS) probes, metallo detectors, acid sensors and enzymatic fluors for glycobiology as well as proteases differentiated from existing probes through a single cleavage to liberate maximum fluorescence will be presented. A series of silicon rhodamine based fluors for super resolution live cell imaging under physiological conditions in microscopy, as well as the corresponding highly photostable fluorescent (Stella Fluor TM) derivatives that have been adapted as labels for cell permeable as well as impermeable applications such as flow cytometry will be described, in particular next generation Ca sensing probes that do not need detergents for permeablization.

4:15-5:15 pm, Theater 2, Learning Center
Horizon Discovery: Gene edited cell models for target validation or disease modeling: the application of gene modulation and modification
Presenter: Daniella Steel
Level: Intermediate
Horizon is at the forefront of gene editing and has a decade of executing over 2,000 gene editing projects, in more than 70 cell lines to provide customer focused solutions. Horizon builds human cell models to broaden the understanding of basic biological pathways, as well as gain knowledge of genetic drivers of diseases and targets for drug discovery. Here we will discuss the application of the latest techniques of gene modulation and modification including 1) an introduction to CRISPR-Cas9 genome engineering, 2) important considerations to take when initiating your gene engineering project, 3) applications of CRISPR-Cas9 cell models, and 4) introduction and applications of CRISPRa and CRISPRi.

5:30-6:30 pm, Theater 2, Learning Center
PerkinElmer: Simple cell-based TR-FRET assays for protein phosphorylation, cell signaling, and biomarker detection
Presenter: Roger Bosse, Ph.D. PerkinElmer
Level: Beginner
Protein phosphorylation is a critical process involved in cell signal transduction. Studies of protein phosphorylation in cells enable the elucidation of complex signaling pathways involved in the progression of many diseases such as cancer, inflammatory diseases, immune diseases, and metabolic diseases. LANCE® Ultra TR-FRET cellular phosphoprotein detection kits provide physiologically-relevant, homogeneous mix-and-read cell signaling and cellular phosophorylation assays that can be measured on the vast majority of plate readers without the need for specially-engineered cell lines. LANCE TR-FRET assays are simple, fast, and easy to optimize in cellular or biochemical formats.

 

8:15-9:15 am, Theater 1, Learning Center
Leica Microsystems Inc.: Visualizing the complexity of life with advanced imaging techniques
Presenter: Jochen Sieber, PhD
Level: Intermediate
Biology is complex and fluorescence microscopy is an important research tool in revealing the details within this complexity. The more insights we gain, the more questions emerge. Two common approaches in unraveling the complexities of biological processes are: 1) resolving relevant structures, and 2) tracking those structures over time. Another emerging trend is functional imaging, which aims to elucidate the function of molecules – not just their localization and abundance. Innovative new imaging solutions using the versatile TCS SP8 confocal platform are featured in this talk. The topics will span discussions of deep intravital multiphoton imaging with the TCS SP8 DIVE´s 4Tune detector and super-resolution imaging through confocal-based and STED methods.

8:15-8:30 am, Theater 2, Learning Center
Infinitesimal LLC: A new paradigm in single-cell transfection and gene editing
Presenter: Vincent Lemaitre
Level: Advanced
Infinitesimal’s NanoFountain Probe Electroporation (NFP-ETM) system for single-cell transfection consists of a robotic arm, with 3-degrees of motion, a microfluidic probe, custom electronics, and software for automated transfection of individual cells. The system is mounted to the body of an inverted fluorescent microscope and interfaced to a PC. By integrating a user interface, the software-controlled robotic arm, and a proprietary cell contact detection algorithm, a “Point-Click-Transfect” paradigm was achieved. Using this system, a library of biomolecules, proteins and nucleic acids were delivered into different adhering cell types such as immortalized and primary cells, e.g., human stem cells, with high efficiency and cell viability. In this presentation we will highlight the NFP-ETM system advantages in applications such as gene editing and cell line generation.

8:30-8:45 am, Theater 2, Learning Center
Lipotype GmbH: Lipotype Shotgun Lipidomics technology for comprehensive, high-throughput lipid analysis
Presenter: Dr. Michal Surma
Level: Advanced
Lipids have been neglected in the ongoing omics revolution. One reason has been the difficulty to comprehensively analyze lipid compositions of different samples – ranging from organs to organelles and bodily fluids – in a true high-throughput mode. Lipotype introduced a mass spectrometry-based approach providing fully quantitative results characterized by high precision and exhaustive lipid coverage. It allows for efficient and convenient analysis of large sample sets, increasing the statistical power of studies and paving the way for making lipidomics a routine tool in biological and clinical research. The successful application of this technology is exemplified by various published studies.

8:45-9:00 am, Theater 2, Learning Center
MiniPCR: Genes in space and on Earth too: DNA technology for all
Presenter: Sebastian Kraves, PhD
Level: Introductory
For decades the tools of modern biology and DNA analysis have remained inaccessible outside of high-end labs. miniPCR develops technology to close this gap, making DNA experimentation accessible to researchers, educators, and extreme locations such as the International Space Station or the Amazon rainforest. In this session we will share the history of miniPCR development and its deployment in various contexts including truffle farming, on-the-ground Ebola diagnosis, and DNA sequencing in space. These examples illustrate how PCR and gel electrophoresis can now be accessed with increased ease and efficiency by researchers, biotechnologists and DNA curious individuals around the globe.

9:00-9:15 am, Theater 2, Learning Center
NanoSurface Biomedical: Biomimetic cell culture platforms for enhancing cell biology studies
Presenter: Nicholas Geisse, PhD
Level: Introductory
Cells maintained in vitro typically exhibit disordered cytoskeletal structures and random orientations. This disordered development can produce aberrant functional profiles and limit the utility of such in vitro models, producing non-predictive data with only limited relevance to in vivo mammalian cell function. Here we demonstrate that extracellular matrix-inspired substrate nanotopography drastically improves the structural and functional development of differentiated cells. Specifically, we show how NanoSurface culture platforms can be utilized to study the effect of cell-nanotopography interactions on adhesion, signaling, polarity, migration, and differentiation in the context of cancer biology, as well as regulation of epithelial wound healing, cardiovascular function, and stem cell biology. This talk will cover a broad range of applications and discuss results achieved from analysis of over twenty adherent cell types.

9:30-10:30 am, Theater 1, Learning Center
3i (Intelligent Imaging Innovations): Living Cell Imaging: Revolutionize Your Research with 3i LightSheet Microscopy
Presenter: Boyd Butler, M.D., Ph.D.
Level: Intermediate
3i (Intelligent Imaging Innovations) pioneers the development and commercialization of a full portfolio of light sheet systems from Lattice LightSheet’s excellent spatiotemporal resolution with minimal photodamage to Cleared Tiling LightSheet’s ability to image whole cleared brains in hours not days. Lattice LightSheet is unparalleled in its ability to achieve both excellent spatial and temporal resolution in cellular samples. Marianas LightSheet offers the ability to image a wide range of specimens with traditional sample preparation and photomanipulation capabilities. Tiling LightSheet images multicellular specimens with high speed and subcellular spatial resolution. Cleared Tiling LightSheet achieves high resolution and unprecedented acquisition speed in large cleared specimens. With these technologies, 3i can provide a complete end-to-end solution for all researchers with either specific or broad imaging needs.

9:30-10:30 am, Theater 2, Learning Center
MilliporeSigma: New advances in microfluidic control of cellular microenvironment with uninterrupted imaging allows for highly controllable, long-term, more in vivo-like cell culture studies
Presenter: Victor Yeh
Level: Intermediate
Performing live-cell experiments within microfluidic chambers greatly extends the precision and biological relevance of in vitro cell culture studies. One major challenge for long-term in vitro culture is controlling and manipulating micro-environmental parameters like temperature and gas composition, without disturbing the culture or impeding optical access to the cells. This workshop will cover the advantages of microfluidic cell culture and live-cell microscopy, and will review cell culture considerations, microfluidic design and fabrication requirements, and integration of microfluidic systems with microscopy for optimum visualization of live cells. Microfluidics applications in fields ranging from bacterial biofilms to tumor metastasis, followed by an introduction to recent microfluidic device designs will also be covered. The workshop will finish with open discussion of applications and future directions of the technology.

10:45-11:45 am, Theater 2, Learning Center
Photometrics: Maximizing sensitivity and signal to noise in scientific imaging
Presenter: Rachit Mohindra
Level: Intermediate
Progress in life science research has benefited from image sensor innovation. This is no more apparent than in scientific CMOS cameras that are the established workhorse solution. Combined with new breakthroughs in computational imaging and signal processing, scientific cameras can move from image capture devices to assisting in selecting and processing important data. A vision for how this is realized is presented. This includes application in super-resolution microscopy and improving signal-to-noise ratios in very low light imaging which is typical of live-cell microscopy. EMCCD sensors previously defined the peak of sensitivity. Today back-side illuminated CMOS sensors are available and poised to eliminate tradeoffs between frame rate, field-of-view and sensitivity. This presentation includes camera performance comparisons and how to select a camera for fluorescence microscopy methods.

12:00-12:45 pm, Theater 1, Learning Center
Wiley: Want people to read your paper? Optimize your chances with the Wiley Researcher Academy
Presenter: Andrew Moore
Level: Advanced
Many people click on but don’t read an article; others don’t even find it. Many sound articles don’t even reach peer review. Which factors influence the success of your manuscript, from submission to publication and final readership recognition? What are editors and reviewers looking for? Did you know that you’re addressing different readerships from submission to publication? How do search engines work, how do editors help authors “optimize” their articles for findability? How has the Internet changed the writing challenge for authors? Increase your understanding of scientific communication, and benefit from insights that you are unlikely to get elsewhere. Opportunity after the talk to learn more about writing and editorial at the Wiley booth.

12:00-12:45 pm, Theater 2, Learning Center
SVI Huygens Software: Restoring light-microscopy images with the SVI-Huygens deconvolution software
Presenter: Dr. Giulia De Luca
Level: Introductory
While analyzing light-microscopy images, you might miss the small features of the sample because the resolution of the microscope is too low or because the images are noisy. This talk will describe how the SVI-Huygens deconvolution software increases more than 2-times the image resolution and more than 10-times the contrast, for a more reliable data visualization and analysis. Join this talk to know how Huygens is: -Comprehensive, addressing drift, chromatic aberration and crosstalk and including visualization and analysis options; -Fast, with its GPU-accelerated algorithms; -Flexible, for beginners with the automatic Deconvolution Express and for advanced users with the Deconvolution wizard, Scripting and Batch processing; -Up-to-date, with the new multi-tile Stitcher wizard and the new Light-sheet Fusion and deconvolution. Info and test: info@svi.nl.

1:00-1:45 pm, Theater 1, Learning Center
Nikon Instruments Inc.: Regulation of microtubule-based motility
Presenter: Samara Reck-Peterson, PhD|
Level: Advanced
In eukaryotic cells, cytoplasmic dynein-1 is essential for long-distance transport of many cargos and can be regulated to perform multiple molecular functions. One essential dynein regulator is Lis1, mutations in which cause the neurodevelopmental disease lissencephaly. I will present our discovery, and the mechanism by which, Lis1 regulates dynein in two distinct and opposing ways. Under some conditions Lis1 causes dynein to tightly bind to its microtubule track, while in other cases it weakens dynein’s microtubule interaction. This work, which uses a combination of single-molecule imaging, in vitro reconstitution and cryo-electron microscopy, provides a new model for how Lis1 can regulate dynein to perform multiple molecular functions.

1:00-1:45 pm, Theater 2, Learning Center
MilliporeSigma: Winning westerns: proven strategies to optimize your western blots
Presenter: Jun Park, PhD
Level: Introductory
Does western blotting give you more trouble than expected? Do you feel like your precious samples are being wasted on bad westerns? Join us and find out how you can improve your western blots! In this seminar, you will learn general guidelines for performing and troubleshooting your westerns, such as: choice of different blotting membranes, parameters affecting blotting efficiency, conditions for optimizing your immunodetection, and information on SNAP I.D. 2.0: a faster way to perform immunodetection. As the inventors of PVDF Immobilon® membranes, MilliporeSigma knows how informative a good western can be. Bring your research questions to get the most out of this session.

2:00-2:45 pm, Theater 1, Learning Center
Cellecta, Inc.: Targeted RNA expression profiling for biomarker discovery in complex samples
Presenter: Paul Diehl, PhD
Level: Intermediate
New rapid and robust transcriptome-based methods for cellular characterization of the tumor microenvironment and biomarker discovery are required to improve prognosis and treatment of cancer and other diseases. However, challenges with current approaches for the above applications include high sample requirements, poor sensitivity, low dynamic range, and limited throughput. To address these limitations, we have developed the Driver-Map targeted RNA expression profiling assay using a genome-wide set of 19,000 validated primer pairs that leverages the sensitivity of multiplex RT-PCR with the throughput and digital readout depth of Next-Generation Sequencing (NGS). We present the performance of the assay for immunophenotyping of immune cells in whole blood samples from sepsis patients and assess the immune responses to complex immunomodulatory stimuli in ex vivo mode.

2:00-2:45 pm, Theater 2, Learning Center
Andor Technology: Practical reasons to consider Dragonfly as your next confocal solution
Presenter: Dr. Mark Browne
Level: Intermediate
Dragonfly is a high performance multi-modal imaging platform. In this sesion, we focus on Dragonfly’s multi-point scanning confocal imaging performance and compare it to single point scanning, which has become the dominant technology over the last 30 years. We show that Dragonfly exceeds or matches the performance of point scanners in all important aspects. As life science research accelerates and demands greater throughput for deeper study, we suggest the community should consider this new and powerful platform wherever there is a need for fast, sensitive, high resolution confocal imaging. Further benefits to your research include greater productivity, extended spectral range, and accurate quantifiable results. From large samples, such as zebrafish and organoids, to single cells or yeast and bacteria, Dragonfly makes no compromises for the best quality image.

3:00-4:00 pm, Theater 1, Learning Center
Berkeley Lights: Smaller is better: isolating and assaying single cells in a nanofluidic chip
Presenter: Mark White, PhD
Level: Intermediate
Smaller is better because it’s faster. Automating biological processes at nano volumes significantly shortens time to results, precisely captures multiple data points on each cell, and scales by screening thousands of single cells at one time. Berkeley Lights’ proprietary OptoSelect™ technology enables precise manipulation of cells, including T cells and B cells, using low-intensity light. For the first time, individual cells can be selectively isolated, cultured, assayed, and exported. Selected cells can also be processed for nucleic acid isolation for further genomic studies. Examples will be shared of identification of cells of interest, assay versatility, and the capture of cell to cell interaction.

3:00-4:00 pm, Theater 2, Learning Center
NemaMetrix Inc.: Functional analysis of disease genes in in vivo systems
Presenters: Dr. Kathryn McCormick and Dr. Chris Hopkins
Level: Intermediate
The age of genomic and precision medicine is revolutionizing medical practices, but it is also revealing gaps in our knowledge of the genetics of disease. Each individual’s genome harbors a significant number of unique alleles that may impact the individual’s health in unpredictable ways. These are the clinical Variants of Uncertain (or Unknown) Significance (VUS). Assigning a functional status to identified alleles, whether benign or pathogenic, is a significant problem. New advances in genome engineering and phenotypic assessment are making it possible to go confidently from identification of a novel patient allele to a ClinVar assignment of variant activity with speed and ease using in vivo functional data. We will show a typical workflow for disease variant analysis, focusing on Epilepsy and Alzheimer’s disease genetics.

4:15-5:15 pm, Theater 2, Learning Center
Nanolive SA: A marker-free technology to analyze living cell’s internal structure and organelles in 3D, at high temporal and spatial resolution
Presenter: Dr. Yann Cotte
Level: Introductory
Holographic-tomographic microscopy is an emerging and powerful new imaging approach that will revolutionize 3D live cell imaging. It allows researchers to characterize cells, organelles and cell dynamics by refractive index variations, with truly no phototoxicity. The 3D Cell Explorer offers a versatile platform for in vitro live cell imaging. It acquires every second a complete 3D image of cells (bacteria, yeast, protozoa, mammalian) and tissues, combined with cellular environment control and fluorescence imaging. Long-term (for weeks) time-lapse imaging enables users to perform continuous quantification of single cells. New hardware and software features offer a complete solution from cellular environment control, over non-invasive 3D image acquisition to post-processing analysis and will be announced in world premiere!

5:30-6:30 pm, Theater 2, Learning Center
Oxford Nanoimaging Ltd.: The Nanoimager: a desktop super-resolution microscope with d-STORM/PALM and SIM functionality
Presenter: Ben Owen
Level: Intermediate
The Nanoimager, the world’s first desktop super-resolution microscope, this year introduces a game-changing development. As first released at ASCB, the Nanoimager from ONI now boasts structured illumination microscopy (SIM) and localization microscopy (d-STORM/PALM) in one desktop device at an astounding new price-point. This talk will discuss the Nanoimager capabilities from super-resolution imaging to single-molecule tracking and single-molecule FRET. The Nanoimager provides unrivaled stability and performance: it is proven in a range of key applications, from tracking exosomes in cells and solution, to visualizing the endocytic process with exceptional resolution. This talk will discuss key features of the Nanoimager that are relevant for cell biologists, provide the first look at the Nanoimager confocal/SIM upgrade and highlight some exciting recent results.

8:15-9:15 am, Theater 1, Learning Center
Leica Microsystems Inc.: Multimodal imaging: the next evolution in advanced live cell research
Presenter: Oliver Schlicker, PhD
Level: Intermediate
Learn how the DMi8 S advanced imaging system from Leica Microsystems enables in-depth analysis of complex dynamic intracellular interactions. Utilizing enhanced hardware and software modules helps drive discoveries into the missing links in your research. Learn how to combine modalities like TIRF, super-resolution, FRAP, photo activation, photo switching, ablation and optogenics into one experiment to gain insight into dynamic processes. This new live cell imaging platform gives you the toolbox to stay on the cutting edge of research by allowing you to see more, see faster and see the hidden.

8:15-8:30 am, Theater 2, Learning Center
Ananda Devices: The future of cell culture models: how microfluidic platforms can increase reproducibility and efficiency of cellular assays
Presenter: Dr. Margaret Magdesian
Level: Introductory
Drug development is slow, expensive and inefficient. Over 60% of biological research cannot be reproduced and scientists still use outdated cell culture models to test new drugs. Major scientific breakthroughs rely on robust cell cultures, reproducible experiments and reliable results. Ananda Devices (www.AnandaDevices.com) bring cell cultures to the 21st century. We offer unique microfluidic devices that enable scientists to reproduce the in vivo environment in vitro. Ananda Devices enable up to 50% faster and more reproducible data analysis in neuroscience, cancer, immunological and iPSC research. We help scientists achieve significant and high-quality results faster.

8:30-8:45 am, Theater 2, Learning Center
AS ONE International, Inc.: AS ONE International, Inc.
Presenter: Hiro Masumoto
Level: Advanced
Established in January 2017, AS ONE International, Inc. is committed to bringing innovative life science technologies from around the world into the hands of every researcher to accelerate discoveries in the areas of molecular and cell biology. With our strong networking in Japan and other markets, we offer unique research instruments and reagents, such as microgravity cell culture device Gravite®, specific proteins and antibodies for DNA 3R research, peptide microarrays for epitope mapping and disease studies, and other innovative research tools for studying cancer, metabolism, neuroscience, stem cell biology, immunology, and signal transduction. AS ONE International is actively seeking potential collaborations to accelerate the growth of the company and its partners.

8:45-9:00 am, Theater 2, Learning Center
Aurox Ltd.: Aurox Clarity LFC: your personal laser free confocal
Presenter: Phillipa Timmins
Level: Intermediate
Based in Oxfordshire, UK, Aurox Ltd. was established to commercialize and build upon pioneering work from the Scanning Optical Microscopy Group at the University of Oxford. The Clarity LFC Laser Free Confocal unit from Aurox is based on a unique design of spinning disc with a grid-like structured illumination pattern. The system provides laser free confocal imaging, setting a new benchmark in price/performance ratio in the field of confocal microscopy. Come along to learn more about the technology and to see how it might complement your research.

9:15-9:30 am, Theater 2, Learning Center
Nanolive SA: A marker-free technology to analyze living cell’s Internal Structure and Organelles In 3d, at High Temporal and Spatial Resolution
Presenter: Nanolive SA
Level: Intermediate
Holographic-tomographic microscopy is an emerging and powerful new imaging approach that will revolutionize 3D live cell imaging. It allows researchers to characterize cells, organelles and cell dynamics by refractive index variations, with truly no phototoxicity. The 3D Cell Explorer offers a versatile platform for in vitro live cell imaging. It acquires every second a complete 3D image of cells (bacteria, yeast, protozoa, mammalian) and tissues, combined with cellular environment control and fluorescence imaging. Long-term (for weeks) time-lapse imaging enables users to perform continuous quantification of single cells. New hardware and software features offer a complete solution from cellular environment control, over non-invasive 3D image acquisition to post-processing analysis and will be announced in world première!

9:30-10:30 am, Theater 2, Learning Center
TESCAN USA, INC.: QPI as a tool for a label-free viability assay with cell death classification
Presenter: Jan Balvan
Level: Intermediate
According to NCCD, a dead cell can be considered a cell which underwent a permanent loss of the barrier function of the plasma membrane or the breakdown into discrete fragments. Quantitative phase imaging is an emerging field of microscopy aimed at studying weakly scattering and absorbing specimens. Integrated phase shift through a cell is proportional to its dry mass, which enables studying cell mass growth or cell mass loss associated with the cell death. Here we are introducing a novel QPI-based method to detect the changes of cell mass in time. This method allows the measurement of viability of cell populations on the basis of the cellular manipulation with its dry mass.

10:45-11:45 am, Theater 2, Learning Center
Semrock (a Division of Idex Health & Science): Striving to meet the new challenges in fluorescence microscopy
Presenters:  Peter Brunt* and Prashant Prabhat
Level: Advanced
This talk reviews the latest developments in fluorescence filters and fluorescence microscopy techniques. With the implementation of super-resolution and multi-photon techniques becoming increasingly commonplace, it is important for filter technologies, which remain at the core of these techniques, to expand and adapt. Both in terms of spectral response and in their physical properties, filters must now account for far more than in original epi-fluorescence techniques and we aim to explore these changes. We will also explore tools that allow users to identify solutions and optimize their experiments. These include SearchLight, an online spectral plotting and analysis tool and MyLight, a powerful means of viewing spectral design responses. *AVR Optics, – Semrock’s distributor for educational institutions and government research labs in US & Canada\

12:00-12:45 pm, Theater 1, Learning Center
MilliporeSigma: Quantifying cell biology through high-speed single-cell imaging and analysis
Presenter: Dr. Darin Fogg
Level: Introductory
This seminar will focus on theory and applications of Amnis imaging cytometry, a unique technology capable of capturing multiple images of single cells at rates of thousands of events per second. Combining the speed and objectivity of flow cytometry with the detailed imagery of microscopy, the ImageStream and FlowSight enable quantitative analysis of cell size and shape, and of fluorescence intensity, texture, location, and co-location. Specific cell biology applications described will be quantification of nuclear-cytoplasmic translocation of molecules, phagocytosis and internalization, autophagy, cell-cell interactions, and characterization of extracellular vesicles.

12:00-12:45 pm, Theater 2, Learning Center
ACEA Biosciences, Inc.: Novel CAR-T Cell platform using xCELLigence® Real-Time Cell Analysis (RTCA), cytokine in vitro assays and in vivo models
Presenter: Dr. Vita Golubovskaya
Level: Intermediate
The novel CAR-T cell platform approaches include 1) CAR-T cells with Decreased Cytokines, 2) Novel CAR co-activation domains, 3) CD47 and humanized CD47-CAR-T cells. Initially, we developed CD19-FLAG-CAR-T cells that significantly blocked Raji tumor burden, as detected by IVIS imaging, and extended mouse survival. CD19-FLAG-CAR-T cells also blocked solid-cancer Hela-CD19 tumor growth. In addition, CD19-FLAG-CAR-T cells secreted significantly less cytokines: IFN-gamma, IL-2 and IL-6. Second, we engineered CAR-T cells with novel co-activation domains (GITR and mutant CD28-domain) with the same RTCA (Real-Time-Cell-Analysis) cytotoxic-activity and less cytokine secretion. The third approach includes generation of novel CD47 and humanized CD47-CAR-T cells against different types of cancer. CD47-CAR-T significantly blocked pancreatic cancer tumor growth using in-vitro and in-vivo models. These novel approaches can be used for future therapies of hematological and solid-cancers.

1:00-1:45 pm, Theater 1, Learning Center
Thermo Fisher Scientific Inc.: New tools for CRISPR gene-editing in cell models and primary blood cells
Presenter: James Kehler, VMD, PhD
Level: Intermediate
The development of the CRISPR/Cas9 gene-editing platform enables the rapid generation of new genetically modified cell models, as well as providing new potential therapeutic treatments. Optimized electroporation and chemical transfection workflows will be presented for efficient co-delivery of Cas9 Ribonucleoprotein complexes made from a next generation TrueCut™ Cas9 Protein v2 with synthetic TrueGuide™ sgRNAs, along with ssDNA templates to perform Homology Directed Repair. Data demonstrating efficient gene-editing in multiple loci across a wide range of cell lines, including traditionally, difficult to manipulate cells such as human iPSC and primary T-cells will be presented. Scientists interested in gene-editing to create primary, pluripotent and cancer cell models for basic research, drug discovery and translational medicine will discover how these new tools can accelerate their research programs.

1:00-1:45 pm, Theater 2, Learning Center
Bruker Corporation: Bruker LUXENDO light-sheet microscopes: pure live imaging across scales
Presenter: Malte Wachsmuth
Level: Intermediate
Light-sheet fluorescence microscopy has become a state-of-the-art imaging method to address a wide variety of biological questions. Featuring extremely low phototoxicity, high-speed image acquisition, and large penetration depth, it allows long-term 3D imaging of large and delicate samples. Fast subcellular processes and interactions can be observed in the comprehensive organ, organoid or organism context. Different samples require different conditions. The LUXENDO product line reflects this fact: the MuVi-SPIM is a horizontal setup designed to image large volumes very fast using two-sided illumination and detection without the need for rotation. The InVi-SPIM is the first inverted light-sheet microscope and is optimized for long-term 3D imaging of delicate samples with full environmental control. The QuVi-SPIM is an upright setup for quantitative systems cell and neurobiology.

2:00-2:45 pm, Theater 2, Learning Center
BD Biosciences: New generation dyes as powerful tools to visualize cell biology in vivo
Presenter: Robert Balderas
Level: Advanced
The advent of brighter and more stable dyes has catalyzed a major breakthrough in our understanding in cell biology. Caveats that usually precluded the visualization of cells in their in vivo habitat have been superseded by the availability of new dyes and polymers, and their enhanced and stable brightness allows the identification of rare cell populations in living organisms. In this talk, we will discuss the rationale and techniques behind the utilization of fluorescence-conjugated antibodies and polymers to perform confocal intravital microscopy of different organs. This allows the visualization in high definition of both tissue architecture and cell populations during health and disease, allowing not only a three-dimensional appreciation of cell-cell interaction, but also dynamic investigation using movie acquisition for long periods. For research use only.

3:00-4:00 pm, Theater 2, Learning Center
Bio-Rad Laboratories: Decoding quantitative western blotting
Presenters: Dr. Poulomi Acharya and Dr. Thomas Berkelman
Level: Intermediate
In the last 40 years, western blotting has become an essential and ubiquitous method for protein and molecular biology research. In the last several years, there have been growing concerns from the scientific community about lack of reproducibility of published data mainly attributed to inconsistent antibody performance. Due to this reproducibility crisis, there is an increasing need for better-qualified, validated reagents and imaging technologies to improve data quality. In this talk we will discuss method optimization, data analysis best practices, and new advancements in technology and reagents that can make western blot data more reproducible and quantitative.