ASCB Rainbow


Subgroup M

Subgroup M: Bottom-Up Cell Biology

1:30 – 5:30 pm, Room 113C
Organizers: Daniel Fletcher, University of California, Berkeley; and Matthew Good, University of Pennsylvania

In vitro reconstitution of biological processes from their component molecular parts is a mainstay of biochemistry and has emerged over the last decade as a powerful tool in cell biology. Recent studies have shown that cell-like structures with micron-scale to millimeter-scale organization can be reconstituted from nanometer-scale parts by combining purified proteins and cytoplasmic extracts with cell-like boundary conditions. By identifying the necessary and sufficient conditions for assembly, these ‘bottom-up’ studies provide new mechanistic insight that complements more traditional ‘top-down’ cell biology. Rapid progress in micropatterning, microfluidics, and microfabrication, coupled with continued advancements in biochemistry and molecular biology, raise the possibility of creating more complete cellular reconstitutions that may one day rival the complexity of live cells and tissues.

Presentations:

1:30-1:35 pm- Introduction: Dan Fletcher, University of California, Berkeley
1:35-1:55 pm- Linking molecular properties to cellular behavior: Adventures in the mesoscopic wilderness. Dyche Mullins, University of California, San Francisco
1:55-2:15 pm- XMAP215 is a microtubule nucleation factor that functions synergistically with the gamma-tubulin ring complex. Akanksha Thawani, Princeton University
2:15-2:35 pm- Self-organization processes in biomolecular systems. Andreas Bausch, Technical University of Munich
2:35-2:55 pm- Reconstituting cytoskeletal self-organization with cell-size confinement. Shin’ ichi Ishiwata, Waseda University
2:55-3:15 pm- Active matters: probing forces, fluctuations and self-organization in actomyosin cortices. Nikta Fakhri, Massachusetts Institute of Technology
3:15-3:35 pm- Dissecting and reconstituting organelle-based nutrient sensing. Roberto Zoncu, University of California, Berkeley
3:40-4:00 pm- Contribution of actomyosin dynamics to TCR clustering and signaling. Mike Rosen, University of Texas Southwestern
4:00-4:20 pm- Quantitative approaches to reconstitute the kinetochore-microtubule interactions. Ekaterina Grishchuk, University of Pennsylvania
4:20-4:40 pm- Stochastic geometry sensing and polarization in a spatially confined lipid kinase-phosphatase competitive reaction. Scott Hansen, University of Oregon
4:40-5:00 pm- Building the mammalian embryo in vivo and in vitro. Meng Zhu, Cambridge University
5:00-5:20 pm- Recapitulating early embryonic spatial patterning. Ali Brivanlou, Rockefeller University
5:20-5:25 pm- Closing remarks. Matt Good, University of Pennsylvania

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