ASCB Rainbow


Subgroup B

Subgroup B:  Advanced Imaging for Quantitative Cell Biology

8:30 am – 12:30 pm, Room 114
Organizers: Melike Lakadamyali, University of Pennsylvania, Perelman School of Medicine; and Jie Xiao, Johns Hopkins School of Medicine

The last decade has seen a revolution in light microscopy where the development of super-resolution microscopy methods shattered the diffraction limit and extended the spatial resolution of light microscopy to the nanometer length scales. We are now in an exciting time in history where the living cell can be visualized with unprecedented resolution in multiple colors and three dimensions. Coupled with imaging modalities that enable visualization of cells in the context of their tissues, these microscopy and nanoscopy methods are bringing about a second revolution in quantitative cell biology. This session will bring together some of the leading experts who have been using and further developing advanced microscopy methods to unravel how cellular organization can regulate cell function.

Presentations:

8:30-8:40 am- Opening Remarks.  Melike Lakadamyali, University of Pennsylvania, Perelman School of Medicine

Session 1: Chair, Melike Lakadamyali, University of Pennsylvania, Perelman School of Medicine
8:40-9:10 am- Analysing nuclear structure by quantitative super-resolution and correlative electron microscopy. Jan Ellenberg, EMBL
9:10-9:40 am-  Using high-throughput super-resolution microscopy to deduce structures and states in cells. Suliana Manley, EPFL
9:40-10:10 am- Imaging the early events of signal transduction. Diane Lidke, University of New Mexico
10:10-10:30 am- Coffee Break

Session 2: Chair, Jie Xiao, Johns Hopkins School of Medicine
10:30-11:00 am- Gene expression by single RNA imaging. Bin Wu, Johns Hopkins School of Medicine
11:00-11:30 am- An update on OligoSTORM and OligoDNA-PAINT. Ting Wu, Harvard Medical School
11:30 am-12:00 pm- A non-enzymatic exponential amplification scheme for RNA detection in situ. Arjun Raj, University of Pennsylvania
12:00-12:30 pm-  Single cell transcriptome and chromosome imaging. Xiaowei Zhuang, Harvard University and HHMI
12:30 pm- Closing remarks. Jie Xiao, Johns Hopkins School of Medicine

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